Skip to main content
Figure 5 | BMC Molecular Biology

Figure 5

From: Multiple tandem splicing silencer elements suppress aberrant splicing within the long exon 26 of the human Apolipoprotein B gene

Figure 5

Activity of key ESS sequences in the context of HßΔ6. (A) The indicated sequences from exon 26 were cloned into the AccI site of exon 2 and tested for in vitro splicing activity (2 hour incubation, 30°C). 5% PAGE shown. Adjacent cartoons show identity of bands. (B) Quantification of splicing efficiency, normalised to HßΔ6 reporter. The bars show splicing efficiency, normalised to HßΔ6 for n=9 experiments, error bars show S.E.M. Overall one-way ANOVA shows p <0.0001. Comparison to HßΔ6 splicing efficiency for each construct by Bonferroni’s multiple comparison test: statistically significant differences shown by **= p <0.001.

Back to article page

BMC Molecular Biology

ISSN: 1471-2199

Contact us