Prediction of potential and experimental target genes associated with up-regulated miRNAs in TIA-depleted HeLa cells. Integrative analyses of potential target genes and miRNAs regulated in TIA-depleted HeLa cells using TargetScan 5.2, PicTar-Vert and miRDB software tools (see corresponding section in Methods). Diagrams depicting the number of potential target genes and up-regulated miRNAs (Additional file 3) by TIA silencing are shown. Venn diagram depicting the numbers of genes that were intersected among putative target genes (highlighted in gray) associated to miRNAs and experimentally defined differentially expressed genes (see additional data files in ) in HeLa cells lacking TIA proteins. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analyses were conducted using software programmes provided by GenCodis3.