Small interfering RNA (siRNA)-mediated depletion of TIA proteins. (A) Western blot analysis of HeLa cell lysates prepared 72 h after transfection with siRNAs against control (C; lanes 1, 3 and 5) and TIA1 plus TIAR (lanes 2, 4 and 6). The blot was probed with antibodies against TIA1, TIAR, and α-tubulin proteins, as indicated. Molecular weight markers and the identities of protein bands are shown. (B) A schematic representation depicting the miRNA array strategy used in this study. The preparation of the RNA samples from control and TIA-depleted HeLa cells, labeling of above RNA samples with Hy3 and Hy5 dyes, hybridization and analyses of the resulting miRNA profiles are illustrated.