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Figure 3 | BMC Molecular Biology

Figure 3

From: UtroUp is a novel six zinc finger artificial transcription factor that recognises 18 base pairs of the utrophin promoter and efficiently drives utrophin upregulation

Figure 3

The trans-activation of the human utrophin promoter “A”. A: Schematic representation of the CJ7-UtroUp chimera in the mammalian expression vector. B: A graphical representation of the human utrophin promoter “A”. The pXP-Luc construct consists of a minimal region of the human utrophin promoter “A” containing the 18-base-pair UtroUp target DNA-binding sequence (indicated in italicised and underlined characters) that was cloned upstream of the reporter gene (Luciferase). C: (Left) A histogram shows the fold of induction of the pXP-Luc utrophin promoter “A” construct or the control reporter pGL2-Promoter (Promega) that was obtained upon co-transfection with the finger region-only UtroUp, or the CJ7-UtroUp or the CJ7-Jazz in HeLa cells. The data are presented as the means ± S.D. of three independent experiments that were performed in triplicate. (Rigth) Western blot analysis cell lysates from HeLa cells transiently cotransfected with pXP-Luc construct and the indicated CJ7-UtroUp or CJ7-Jazz expression vectors. The membrane was incubated with the anti-myc tag antibody and the anti-alpha tubulin antibody.

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