Skip to main content
Figure 7 | BMC Molecular Biology

Figure 7

From: Dissecting domains necessary for activation and repression of splicing by muscleblind-like protein 1

Figure 7

PTB co-regulates Tpm1 but not Vldlr splicing. A. Western blots with anti-PTB (upper), anti-FLAG (middle) and anti-actin (lower panel). Cells were treated with control C2 siRNA (lanes 1–6) or PTB/nPTB siRNAs (lanes 7–9). Lanes 4–1 show successive 2-fold dilutions of the C2 control to allow assessment of knockdown. In lanes 6 and 9, FLAG-PTB was overexpressed; in lanes 5 and 8, FLAG-MBNL1 was overexpressed. B. The Tpm1 ΔBP minigene was cotransfected with control C2 (lanes 1–3) or PTB/nPTB siRNAs (lanes 4–6). In addition, FLAG-PTB (lanes 3, 6) or FLAG-MBNL1 (lanes 2, 5) were also cotransfected. C. The Vldlr minigene was cotransfected with control C2 (lanes 1–3) or PTB/nPTB siRNAs (lanes 4–6). In addition, FLAG-PTB (lanes 3, 6) or FLAG-MBNL1 (lanes 2, 5) were also cotransfected.

Back to article page