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Table 2 Oligonucleotides used to create rtMT-A gene promoter deletion mutants

From: Differential regulation of the rainbow trout (Oncorhynchus mykiss) MT-A gene by nuclear factor interleukin-6 and activator protein-1

Primer

Location

Sequence*

NF-IL6 forward

(-1042 to -1020)

GCG GGT ACC TAT GTT CGA TTG GAC TAT GAT TC

AP1 forward 1

(-939 to -917)

GCG GGT ACC TGA TAG ACT ATC CTT GTT GTA GG

AP1 forward 2

(-834 to -815)

GCG GGT ACC ATA ACA TTG CAC AAT GTT TG

MRE forward

(-793 to -773)

CGG GGT ACC TCA AGC AGG AGA TTC TGG AA

NF-IL6 reverse

(917 to -939)

GCG AAG CTT TTT ATA TCG CTA CAA TTA ATT ACA AAC GAC CG

AP1 reverse 1

(-774 to -790)

GCG AAG CTT TTT ATA TCG CCA GAA TCT CCT GCT TG

AP1 reverse 2

(-812 to -829)

GCG AAG CTT TTT ATA TCG CCA CAA ACA TTG TGC AAT G

MRE reverse

(+23 to +5)

GCG AAG CTT CAG TGG TGT GTT GTC AGC G

  1. *The restriction enzyme sites are shown in bold (the 3′ primers contain a Hind III site and the 5′ primers contain a Kpn I site) and TATAA box sequences are shown in italics. The MRE contains the natural TATAA box found in rtMT-A.