Figure 6

Activation of rtMT-A deletion constructs following exposure to PQ. RTH-149 cells were transfected with 0.5 ug of MRE-AP1-NFIL6-pGL3, MRE-AP1-pGL3 and MRE-pGL3 vectors and 0.3 μg of pRL-CMV vector. Following transfection cells were exposed to 10 μM PQ. The activities are given as fold induction. All activities were normalized to the expression level of the pGL3-basic vector. Luciferase activities were analyzed 24 hours post transfection. Results are presented as mean ± SE (n = 4). Statistically significant differences from control levels are indicated by *(p < 0.05); **(p < 0.01).