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Figure 5 | BMC Molecular Biology

Figure 5

From: Differential regulation of the rainbow trout (Oncorhynchus mykiss) MT-A gene by nuclear factor interleukin-6 and activator protein-1

Figure 5

In vitro competition assay. Hep G2 cells were transfected with the 4AP1-pGL3 vector (AP1tot) (A) or 6MRE-4AP1-pGL3 vector (MRE-AP1) (B) alone or together with 50 x molar excess of the AP11-2 and AP13-4 oligonucleotides. Control groups incubated without exposure (Control vs. Control-Comp), while experimental cells were exposed to 200 μM H2O2 (H2O2 vs. H2O2 Comp). Results are presented as mean ± SE (n = 4). Statistically significant differences between groups are indicated by *(p < 0.05); **(p < 0.01).

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