Figure 1

Cis-acting elements of the rtMT-A promoter. The location and orientation of the NF-IL6, AP1 and MRE elements on the rtMT-A enhancer region is shown. There are 6 MREs (MRE), 4 AP1 (AP1_tot) and 1 NF-IL6 (NF-IL6) element located in the first 1100 bp upstream of the transcriptional start site in the rtMT-A gene. The constructs shown are -1042/-917 that contain the NF-IL6 element alone; -939/-812 that contain AP1_3,4; -834/-774 that contain AP1_1,2; -939/-774 (AP1_tot); -793/+23 (MRE); -939/+23 (MRE-AP1); -1042/+23 (MRE-AP1-NFIL6). The TATA box found in rtMT-A is included in the reverse primers of the distally located constructs. The constructs were cloned into the pGL3-basic vector.