Figure 4
From: MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation
Sustained expression of MAR-containing expression vectors. (A, B) Mice muscles were electrotransferred with EPO-expressing network plasmids containing or not hMAR 1–68, and the blood plasma of mice treated with doxycycline or not was used to quantify EPO levels by ELISA. (C) Mice similarly treated as those of panels A and B were sacrificed 5 or 25 days post-electrotransfer, and total DNA was extracted from the electrotransferred muscles. Episomal plasmid rescue was performed by transforming competent bacteria with a fraction of the total DNA extract and by scoring the occurrence of ampicillin-resistant colonies on Petri dishes.