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Table 1 Expression data for candidate reference genes from Pseudo-nitzschia multiseries ( Ps-n ) cDNA microarray analysis a

From: Gene expression studies for the analysis of domoic acid production in the marine diatom Pseudo-nitzschia multiseries

   Fold changeb  
Stationary versus late-exponential phase
Ps-n NR Identifier JGIPs-n Genome hit Non-axenic Non-axenic Axenic Predicted gene product
  Scaffold:Start-End Expt. 1 Expt. 2 Expt.  
53B6 41:203449-205143 1.05 ± 0.01 1.04 ± 0.05 1.05 ± 0.03 JmjC-domain family protein (JmjC)
45E3 55:316954-329784 1.10 ± 0.02 1.24 ± 0.07 1.36 ± 0.05 Dynein heavy chain, cytosolic
177F1 198:180888-181958 1.05 ± 0.02 0.93 ± 0.01 0.81 ± 0.01 Histone H3
PSN0918 2485:4610-6293 1.30 ± 0.00 1.29 ± 0.37 0.99 ± 0.01 Cyclophilin
PSN0001 10:398258-400584 1.37 ± 0.10 1.19 ± 0.07 1.10 ± 0.17 Elongation factor 1-alpha (EF-1α)
PSN0547 210:148023-149837 0.90 ± 0.08 0.78 ± 0.05 1.26 ± 0.03 Phosphoglycerate kinase (PGK)
PSN1327 890:26709-27681 1.00 ± 0.03 1.07 ± 0.00 1.24 ± 0.03 Elongation initiation factor 2 (eIF-2)
PSN0332 2:525315-527491 1.13 ± 0.05 1.29 ± 0.03 1.19 ± 0.17 ATPase with AAA domain
PSN0032 18:343323-346000 0.74 ± 0.04 0.68 ± 0.06 0.44 ± 0.03c Ubiquitin
PSN1138 68:114178-115516 0.87 ± 0.08 0.92 ± 0.09 1.73 ± 0.23c Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
  1. aPotential reference genes for RT-qPCR studies were selected based on their stability in the microarray results or their use as controls in previous studies.
  2. bThe fold-change data presented in Table 1 represents the average differences across all of the cDNA clones that were printed on the array for each transcript. As follows, the number of independent cDNA clones for each transcript was: 53B6 (1), 45E3 (1), 177F1 (1), PSN0918 (1), PSN0001 (58), PSN0547 (2), PSN1327 (1), PSN0032 (7), PSN0332 (2), PSN1138 (6). Each clone was printed in duplicate on array. 18s not printed on array.
  3. cTranscript levels showed statistically significant differences between samples.