Figure 3

RT-qPCR analysis of candidate reference genes for normalization from Pseudo-nitzschia multiseries . Bars represent the mean change (± 1 SD) in expression relative to Day 3. Open bars represent measurements using primers that spanned an exon-exon junction; grey bars represent measurements using standard primers that did not span an exon-exon junction. Means were not significantly different (p < 0.05), except EF-1α. Means with different letters were significantly different (p < 0.05). Statistical analyses were performed using a general linear model ANOVA with Bonferroni post-hoc test, 95% confidence intervals.