Figure 2

Average expression stability (M-value) of the reference genes determined by GeNorm analysis. An individual reference gene is tested against the other reference genes in a pairwise variation that serially excludes the least stable genes from the analysis. The most stable reference genes exhibit the lowest M-values. The accepted cut-off for stability of reference genes is an M-value of 0.50. a) In the initial analysis, which tested a subset of mRNA samples under Si-limited conditions for all eleven of our potential reference genes, four reference genes were determined to be acceptably stable. b) In the analysis of the Si-limited growth experiment, these four reference genes and EF1-alpha were tested. All five showed acceptable stability across the mRNAs, and JmjC, Dynein, and Histone H3 were tied for the 1st rank.