Figure 1
From: 3′ terminal diversity of MRP RNA and other human noncoding RNAs revealed by deep sequencing
3′ RACE with deep sequencing, schematic of method used. a Ligation of whole cell RNA with 5′ degenerate indexed appendices (orange), b Reverse transcription with appendix-specific primer, c 3′ RACE-PCR selection with indexed (black and white barcode) gene-specific F primer and universal R primer containing Illumina adapter sequences (blue), d Library amplification with general primers to the adapter sequences, e High-throughput insert and RACE index sequencing on Illumina MiSeq (sequencing primers in purple), f Bioinformatic trimming of reads to analyze 3′ terminal sequences (yellow). See Methods for detailed protocol.