Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells

BMC Molecular Biology

Table 1 Reference loci and target genes included in the experiments

Reference	Name	NCBI RefSeq	Function	Amplicon size (bp)	Exon location
ACTB^*	Beta-Actin	NM_001101	Cytoskeletal protein	173	7p22
B2M^§	Beta-2-microglobulin	NM_004048	Major histocompatibility complex I component	86	15q21-q22
GAPDH^*	Glyceraldehyde-3-phosphate dehydrogenase	NM_002046	Oxidoreductase in glycolysis and gluconeogenesis	111	12p13
HMBS^*	Hydroxymethyl-bilane synthase	NM_000190	Porphyrin metabolism and heme synthesis	64	11q23
HPRT1^*	Hypoxanthine phosphoribosyl-transferase 1	NM_000194	Purine synthesis in salvage pathway	94	Xq26
PPIA^*	Peptidylprolyl isomerase A (Cyclophilin A)	NM_021130	Catalyzation of cis-trans isomerization of proline imidic peptide bonds	71	7p13
RPL13A^*	Ribosomal protein L13A	NM_012423	60S ribosomal subunit structural component	126	19q13
SDHA^*	Succinate dehydrogenase complex, subunit A	NM_004168	Electron transporter in citric acid cycle and respiratory chain	86	5p15
TBP^*	TATA box binding protein	NM_003194	General transcription factor for RNA polymerase II	89	6q27
UBC^*	Ubiquitin C	NM_021009	Involved in protein degradation	133	12q24
YWHAZ^§	Tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein, zeta polypeptide	NM_003406	Signal transduction; binds to phosphorylated serine residues on several signaling molecules	94	2p25
AluSq	Alu repeats, subfamily Sq
POU5F1^§	POU class 5 homeobox 1, Oct4	NM_002701.4	Marker for embryonic stem cell pluripotency	130	6p21
NANOG^*	Nanog homeobox	NM_024865.2	Marker for embryonic stem cell pluripotency	109	12p13

^*exon-spanning primer pair; ^§forward and reverse primer localized in the same exon.

ISSN: 1471-2199