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Table 1 Reference loci and target genes included in the experiments

From: Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells

Reference Name NCBI RefSeq Function Amplicon size (bp) Exon location
ACTB* Beta-Actin NM_001101 Cytoskeletal protein 173 7p22
B2M§ Beta-2-microglobulin NM_004048 Major histocompatibility complex I component 86 15q21-q22
GAPDH* Glyceraldehyde-3-phosphate dehydrogenase NM_002046 Oxidoreductase in glycolysis and gluconeogenesis 111 12p13
HMBS* Hydroxymethyl-bilane synthase NM_000190 Porphyrin metabolism and heme synthesis 64 11q23
HPRT1* Hypoxanthine phosphoribosyl-transferase 1 NM_000194 Purine synthesis in salvage pathway 94 Xq26
PPIA* Peptidylprolyl isomerase A (Cyclophilin A) NM_021130 Catalyzation of cis-trans isomerization of proline imidic peptide bonds 71 7p13
RPL13A* Ribosomal protein L13A NM_012423 60S ribosomal subunit structural component 126 19q13
SDHA* Succinate dehydrogenase complex, subunit A NM_004168 Electron transporter in citric acid cycle and respiratory chain 86 5p15
TBP* TATA box binding protein NM_003194 General transcription factor for RNA polymerase II 89 6q27
UBC* Ubiquitin C NM_021009 Involved in protein degradation 133 12q24
YWHAZ§ Tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein, zeta polypeptide NM_003406 Signal transduction; binds to phosphorylated serine residues on several signaling molecules 94 2p25
AluSq Alu repeats, subfamily Sq
POU5F1§ POU class 5 homeobox 1, Oct4 NM_002701.4 Marker for embryonic stem cell pluripotency 130 6p21
NANOG* Nanog homeobox NM_024865.2 Marker for embryonic stem cell pluripotency 109 12p13
  1. *exon-spanning primer pair; §forward and reverse primer localized in the same exon.