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Table 1 Primers used in RT-PCR, 5’ and 3’ RACE, synthesizing dsRNA, and performing qRT-PCR

From: Molecular cloning and RNA interference-mediated functional characterization of a Halloween gene spook in the white-backed planthopper Sogatella furcifera

Primer

Sequence (5’ to 3’)

Amplicon size (bp)

Primers used in RT-PCR

  spoFp

ACGGCCAGTCCATTTCAG

344

  spoRp

TGTTGGATGAGGCAGTCG

Primers used in 5’-RACE

  spoGSP

GATGCCAAGTGAGGGTGGGATC

 

  spoNGSP

TCGGTGAAGTCTTTGGGCTCGG

 

Primers used in 3’RACE

  spoGSP

ACGCGACATTCGCCGCTTTGTG

 

  spoNGSP

CGATGCGCTGCTCGCTCACCTT

 

Primers used in PCR for End to End

  spoFp

CGTCGTGAACACCCTTAT

2090

  spoRp

GCCCGGTACACTATTATCTT

 

Synthesizing the dsRNAs

  spoFd

CCCTCACTTGGCATCAC

415

  spoRd

TCGGGTTTCTTTATTTGTC

  egfpup

AAGTTCAGCGTGTCCG

414

  egfpdown

CTTGCCGTAGTTCCAC

Performing the qPCR

  spoFq

CAACTCCATACACATCAAGCCACTG

119

  spoRq

ACCGACGCACCATATCTCTGAAC

 

  EcRFq

AATGAGTTCGAGCACCCTAGCGAA

129

  EcRRq

AATGGTGATTTCGGTGATGTGGCG

 

  RPL9Fq

TGTGTGACCACCGAGAACAACTCA

131

  RPL9Rq

ACGATGAGCTCGTCCTTCTGCTTT

 

  ARFFq

CACAATATCACCGACTTTGGGATTC

141

  ARFRq

CAGATCAGACCGTCCGTACTCTC

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