Primer | Sequence (5’ to 3’) | Amplicon size (bp) |
---|---|---|
Primers used in RT-PCR | ||
  spoFp | ACGGCCAGTCCATTTCAG | 344 |
  spoRp | TGTTGGATGAGGCAGTCG | |
Primers used in 5’-RACE | ||
  spoGSP | GATGCCAAGTGAGGGTGGGATC |  |
  spoNGSP | TCGGTGAAGTCTTTGGGCTCGG |  |
Primers used in 3’RACE | ||
  spoGSP | ACGCGACATTCGCCGCTTTGTG |  |
  spoNGSP | CGATGCGCTGCTCGCTCACCTT |  |
Primers used in PCR for End to End | ||
  spoFp | CGTCGTGAACACCCTTAT | 2090 |
  spoRp | GCCCGGTACACTATTATCTT |  |
Synthesizing the dsRNAs | ||
  spoFd | CCCTCACTTGGCATCAC | 415 |
  spoRd | TCGGGTTTCTTTATTTGTC | |
  egfpup | AAGTTCAGCGTGTCCG | 414 |
  egfpdown | CTTGCCGTAGTTCCAC | |
Performing the qPCR | ||
  spoFq | CAACTCCATACACATCAAGCCACTG | 119 |
  spoRq | ACCGACGCACCATATCTCTGAAC |  |
  EcRFq | AATGAGTTCGAGCACCCTAGCGAA | 129 |
  EcRRq | AATGGTGATTTCGGTGATGTGGCG |  |
  RPL9Fq | TGTGTGACCACCGAGAACAACTCA | 131 |
  RPL9Rq | ACGATGAGCTCGTCCTTCTGCTTT |  |
  ARFFq | CACAATATCACCGACTTTGGGATTC | 141 |
  ARFRq | CAGATCAGACCGTCCGTACTCTC |  |