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Figure 2 | BMC Molecular Biology

Figure 2

From: Molecular cloning and RNA interference-mediated functional characterization of a Halloween gene spook in the white-backed planthopper Sogatella furcifera

Figure 2

Graphic representation of the relative Sfspo transcript levels measured in the whole bodies of second-, third- and fourth-instar (I2D1, I2D2, I3D1, I3D2, I4D1, I4D2 and I4D3) nymphs at 24 h intervals (A) and the head, thorax and abdomen of day 3 fourth-instar nymphs (B). For each sample, 3 independent pools of 5–10 nymphs were measured in technical triplicate using qRT-PCR. The values were calculated using the 2-ΔΔCt method. The relative expression levels were the ratios of relative copy numbers in individuals of specific developmental stage or specific body part to that in I2D1 or head. The columns represent averages with vertical bars indicating SE.

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BMC Molecular Biology

ISSN: 1471-2199

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