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Figure 3 | BMC Molecular Biology

Figure 3

From: Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus

Figure 3

DNA, amino acid sequence and functional motifs of the tsoIRM gene and its flanking regions and schematic organization of the enzyme domains. The predicted amino acid sequence of the 127.6 kDa TsoI protein is indicated in capital letters. The DNA sequence is numbered in two styles: (i) black numbering starts with negative values, with +1 nt marking the beginning of the tsoIRM ORF, (ii) the blue numbering corresponds to the entire DNA sequence deposited in the GenBank under acc. number KC503938] (includes tsoIRM gene and flanking regions). The ATG START codon is in black bold. The TGA stop codon is shown in red. The potential tsoIRM ORF Ribosome Binding Sites (RBS) are boxed. The potential internal ATG START is in blue. The potential tsoIRM internal ORF RBS is boxed in blue. The crucial amino acids of the catalytic centres are dark red, bold and underlined. The functional protein domains are marked as follows: REase domain in blue, helical domain in light green, MTase domain in dark green, TRD in brown.

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BMC Molecular Biology

ISSN: 1471-2199

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