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Figure 2 | BMC Molecular Biology

Figure 2

From: Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus

Figure 2

Evaluation of resistance to TsoI digestion of plasmids, obtained after two rounds of biochemical selection. Lanes M – DNA standards (Gene Ruler™ Ladder Mix (Thermo Fisher Scientific (Fermentas); selected bands marked); lanes 1, 4, 7, 10 and 13 - DNAs of individual plasmids, untreated; lanes 2, 5, 8, 11 and 14 - DNAs of individual plasmids treated with TsoI; lanes 3, 6, 9, 12 and 15 - DNAs of individual plasmids supplemented with λ DNA and treated with TsoI. Samples were resolved on 1.2% agarose gel in TBE buffer, stained with EtBr.

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