Requirement of YB-1 domains for its binding to Tip110. A. Scheme of YB-1 and its deletion mutants used in this study. A/P: Alanine/Proline rich; CSD: cold chock domain; B/A: basic/acidic repeats. B. 293T cells were transfected with pTip110-HA and pYB-1-Myc, or one of YB-1 mutants. Cell lysates were directly used for Western blotting using anti-Myc (top panel) or anti-Tip110 antibody (middle panel), or immunoprecipitated using anti-HA antibody, followed by Western blotting using anti-Myc (bottom panel). The long exposure of the area between the dotted lines was shown at the very bottom panel for the band marked by an arrowhead. #: Reactive IgG.