Requirement of Tip110 domains for its binding to YB-1. A. Scheme of Tip110 and its deletion mutants used in this study. HAT: half-a-tetratricopeptide repeat; NLS: nuclear localization signal; RRM: RNA recognition motif. B. 293T cells were transfected with pTip110-HA or each of Tip110 mutants. Cell lysates were directly used for Western blotting using anti-Tip110 antibody (top and middle panels), or immunoprecipitated using anti-YB-1 antibody, followed by Western blotting using anti-Tip110 (bottom panels). *: Expected proteins. #: Reactive IgG.