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Table 4 Results of the assignments after discriminant analysis

From: How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population

Genes included

Grouping variable

Colour

Colour (no white)

Intensity

W

Q

CHS/F3H/F3′H

40.3%

59.7%

81.6%

59.7%

51.1%

CHS/F3H/F3′H/FLS

51.4%

65.3%

85.7%

58.3%

68.1%

DFR/ANS

27.8%

32.7%

57.1%

55.6%

51.1%

All genes

52.8%

55.1%

81.6%

55.6%

63.8%

  1. Log-transformed CNRQ values of a combination of genes was used to calculate a discriminant function to predict classification according to 5 classes: colour (white, red, carmine red or pink); colour (no white; only red, carmine red and pink); intensity (pink versus (carmine) red); W (coloured versus white) and Q (co-pigmentation versus no co-pigmentation). The percentage of correctly assigned samples is presented.