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Figure 6 | BMC Molecular Biology

Figure 6

From: Identification of nucleotides and amino acids that mediate the interaction between ribosomal protein L30 and the SECIS element

Figure 6

Point mutations in L30 affect RNA-binding activity. A. Representative REMSA analysis of the 32P-labeled PHGPx SECIS (top panel) or L30 RNA (bottom panel). The RNA probes were incubated in the absence or presence of wild-type and mutant L30 proteins as indicated. The positions of the free probes and protein:RNA complexes are indicated. B. Graphical representation of REMSA results for L30 binding to the SECIS (top panel) or L30 RNA (bottom panel) from 5 or 3 independent experiments, respectively. The results are expressed relative to the activity of the wild-type protein, which is expressed as 100%. Statistical significance is shown by asterisks, with * (p < 0.05), ** (p < 0.01) and *** (p < 0.001).

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