Table 1 Primers used in the present study
Primer name | Primer sequence (5’ to 3’) | Purpose |
|---|---|---|
Stra8-F1 | CCAACATCCAGGAGTTTGA | cDNA fragment PCR |
Stra8-F2 | GGGATATCTCTTTTTCTAC | |
Stra8-F3 | GCCATCTCAGGCCTGTGG | |
Stra8-R3 | CCTGAGATGGCCTCGGACACTAC | |
Stra8-R2 | CTGTAGTAGATCCTCCTCA | |
Stra8-R2 | CACCACATCATAGGCATCCTG | |
Stra8-RACE-F1 | GAGCTTCTCTTGCGCAGTGGAGTG | RACE PCR |
Stra8-RACE-F2 | GTCCGAGGCCATCTCAGGCCTGTGGA | |
Stra8-RACE-F3 | CCACTGACTCACATCTCTACGGCTT | |
Stra8-RACE-R3 | CACTCCACTGCGCAAGAGAAGCTCGA | |
Stra8-RACE-R2 | CACAGGCCTGAGATGGCCTCGGACACT | |
Stra8-RACE-R1 | GCCGTAGAGATGTGAGTCAGTGGTCT | |
Stra8-sF0 | CCTCAGCAAGCTCCTTGCACTGAA | Tissue distribution |
Stra8-sR0 | GCTCTGCAGTACTGAAGGCCTGTT | |
Stra8-F0 | GAGCAGGTATGTCCCGTTTGG | Full length cDNA amplification |
Stra8-R0 | GGAGGAACTGGCTAGAAATCC | |
Stra8-Q-F | AGTGCCTGATGTAGATGA | Real-time PCR |
Stra8-Q-R | AGAGTCTCGCTGTAGAAG | |
sc-β-actin-F | GGCATCACACCTTCTACAACGA | Internal control |
sc-β-actin-R | ACGCTCTGTCAGGATCTTCA |