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Table 2 Primer names and restriction sites used to construct the expression vectors

From: Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus)

Name

Restriction site

Vector

AKI-F-A7

SalI

pGBKT7-PoAkirin1

AKI-T-A1

KpnI

AD-AKI-S1

EcoRI

pGADT7-PoAkirin1

AD-AKI-A1

XhoI

C1q-S1

EcoRI

pGBKT7- PoC1q

C1q-A1

SalI

HEPN-S3

EcoRI

pGADT7- PoHEPN-M1

HEPN-A2

XhoI

HEPN-S3

EcoRI

pGADT7- PoHEPN-M2

HEPN-A3

XhoI

HEPN-S5

EcoRI

pGADT7- PoHEPN-M3

HEPN-A4

XhoI

HEPN-ET-S1

EcoRI

pET30a-PoHEPN

HEPN-ET-A1

XhoI