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Table 2 Primer names and restriction sites used to construct the expression vectors

From: Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus)

Name Restriction site Vector
AKI-F-A7 SalI pGBKT7-PoAkirin1
AKI-T-A1 KpnI
AD-AKI-S1 EcoRI pGADT7-PoAkirin1
AD-AKI-A1 XhoI
C1q-S1 EcoRI pGBKT7- PoC1q
C1q-A1 SalI
HEPN-S3 EcoRI pGADT7- PoHEPN-M1
HEPN-A2 XhoI
HEPN-S3 EcoRI pGADT7- PoHEPN-M2
HEPN-A3 XhoI
HEPN-S5 EcoRI pGADT7- PoHEPN-M3
HEPN-A4 XhoI
HEPN-ET-S1 EcoRI pET30a-PoHEPN
HEPN-ET-A1 XhoI