Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder ( Paralichthys olivaceus )

Table 1 Oligonucleotide primers used in this study

Name	Sequence	Purpose used
AKI-F-S1	ATGGCCTGCGGAGCGACGTT	ORF amplification
AKI-F-A1	TCAGGAGACATAACTAGCAGGCCG	ORF amplification
β-actin-s1	GCTGTGCTGTCCCTGTA	RT-PCR
β-actin-a1	GAGTAGCCACGCTCTGTC	RT-PCR
GAP-S1	CAACGGCGACACTCACTCCTC	RT-PCR
GAP-A1	TCGCAGACACGGTTGCTGTAG	RT-PCR
TUBA-S1	TGACATCACAAACGCCTGCTTC	RT-PCR
TUBA-A1	GCACCACATCTCCACGGTACAG	RT-PCR
AKI-R-S1	AGGACCAGCCCTCGTTCACACT	RT-PCR
AKI-R-A1	TCCGTATCTTCGCATGATCTGGT	RT-PCR
HEPN-R-S1	TACAAGGACAATGGTGGGGG	RT-PCR
HEPN-R-A1	GGCAAGGGCTGAGATGGAG	RT-PCR
C1q-R-S1	CTCCAGAAAACGAAGCAGGC	RT-PCR
C1q-R-A1	TGTCGCACATCATCAAGTGAAC	RT-PCR
AKI-F-A7	GCGTCGACGGAGACATAACTAGCAG	Plasmid construction
AKI-T-A1	GCGGTACCGGAGACATAACTAGCAG	Plasmid construction
AD-AKI-S1	GTGAATTCATGGCCTGCGGAGCGACGTT	Plasmid construction
AD-AKI-A1	CAGCTCGAGTCAGGAGACATAACTAGCAG	Plasmid construction
C1q-S1	GCCGAATTCAAGGGGGCACCAGGTCTTAA	Plasmid construction
C1q-A1	CAGGTCGACTCAGGCCGTGGGGAAGACGA	Plasmid construction
HEPN-S3	GTGAATTCGGCACGAGGCTCAGGTGGCA	Plasmid construction
HEPN-A2	CAGCTCGAGAGCCTCCTCTTTGTTTGGCC	Plasmid construction
HEPN-A3	CAGCTCGAGGGAAGGTTGACCGTGCCTTT	Plasmid construction
HEPN-S5	AGTGAATTCTCTGGCAGCTCCATCTCAGC	Plasmid construction
HEPN-A4	CAGCTCGAGCTATTTCACATATGCCTCAAC	Plasmid construction
HEPN-ET-S1	CCGAATTCAGGCTCAGGTGGCATCCT	Plasmid construction
HEPN-ET-A1	GTGCTCGAGCTATTTCACATATGCCTCA	Plasmid construction