Quantitative RT-PCR analyses of the expression level of PoAkirin1 and PoHEPN mRNA in the spleen (A), liver (B), and kidney (C) after injection with Vibrio anguillarum . Figures show the relative expression levels of PoAkirin1 and PoHEPN at 6 h, 12 h, 24 h, 48 h, 72 h and 96 h post-injection. CK-M represents the tissues at 12 h after saline solution injection, and CK means live fish under normal conditions. Data are expressed as the mean ± S.D. (n = 3). Significant differences are indicated with an asterisk at P < 0.05 and two asterisks at P < 0.01.