Figure 2

Loss of MOF causes asynchronous cell cycle, mitotic catastrophe and nuclear fallout. Early embryos (0–2 h) of yw^67c23, mof¹ and mof³ were collected, fixed with DNA dye PI and visualized using confocal microscopy. (A) Large fragmented nuclei indicating the occurrence of mitotic catastrophe is seen in the early embryos of mof¹and mof³ mutants when compared to control embryos. (B &C) Increased number of fall out nuclei is observed in the mof¹ and mof³ mutants when compared to control yw^67c23 embryos. Bar indicates 10 μm scale. The data is represented in the form of bar diagram. Statistical significance was assessed using student t-test. *** indicates P<0.001, ** indicates P<0.01, * indicates p<0.05.