Haplo-insufficiency of mof1heterozygotes. Total protein was isolated from early embryos of control (yw67c23), mof 1and mof 3 (1–2 h) and western blot analysis carried out using mof antibody has shown 3-fold reduction in the maternal MOF protein. β-actin is used as an internal loading control. Statistical significance was assessed using student t-test. *** indicates P<0.001, ** indicates P<0.01, * indicates p<0.05.