Figure 2

Western blot results. 3T3-L1 cells were differentiated with insulin, dexamethasone and IBMX. Protein was isolated from whole cell extracts 0 h (T0) and 6 h (T6) after hormonal induction. 30 μg of each sample was subjected to Western blot analysis for eIF4B, IMPDH2, RPL27a and UBE2k/HIP2 expression. Changes in protein expression were quantified by densitometry and normalized with appropriate expression data of β Actin. The values represent the average of three independent experiments, and the asterisk denotes a p-value < 0.05. eIF4B and RPL27a protein expression is 1.4 fold higher (p-value < 0.05) at T6 than time point T0. IMPDH2 and UBE2k/HIP2 show no significant differences in protein expression between T0 and T6.