Figure 1
From: EGF activates TTP expression by activation of ELK-1 and EGR-1 transcription factors
(A) TTP mRNA expression upon EGF stimulation. Serum-starved MCF-7 cells were stimulated with EGF (20 ng/ml) for indicated times. The total RNA was extracted and analyzed by Real Time PCR using ZFP36 specific primers. (B) Activation of ERK1/2 pathway upon EGF stimulation. Serum-starved MCF-7 cells were stimulated with EGF (20 ng/ml) or PMA (100 ng/ml) for indicated times, harvested and subjected to Western blot analysis (with anti-phospho ERK1/2, anti-ERK1/2, anti-phospho p38, anti-p38, anti-phospho JNK and anti-JNK antibodies)