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Figure 4 | BMC Molecular Biology

Figure 4

From: Protein kinase CK2 localizes to sites of DNA double-strand break regulating the cellular response to DNA damage

Figure 4

CK2 depletion results in decreased DNA-PKcs phosphorylation in M059K cells following induction of DNA double-strand break. A. Cells were treated as described in Figure 1. Whole cell extracts were employed for Western blot analysis of the indicated proteins. Detection of β-actin was used as loading control. B. Cells were treated essentially as described in Figure 1 except that DNA damage was induced by cell exposure to 10 Gy ionizing radiation. After 1 hour, cells were harvested and total cell lysates were prepared for Western blot analysis. C. M059K cells were treated with 33 μM cisplatin (cisPt) or exposed to 20 J/m2 UV irradiation for the indicated time-points. Cell extract from control and treated cells was prepared and analyzed by Western blot employing antibodies against DNA-PKcs or its phosphorylated forms as indicated in the figure. D. Experiments were performed as described above and in Figure 1 except that cells were treated with cisPt (33 μM, 8 hours incubation) or exposed to UV (20 J/m2, 8 hours recovery) as indicated in the figure. Whole cell lysates were prepared for the analysis by Western blot of DNA-PKcs phosphorylation at S2056, T2609 and T2647. Experiments were performed at least three times obtaining similar results. Data from one representative experiment are shown.

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