Table 1 The primers for site-directed mutation PCR

E2-up

5'AAGCTTATGGAAACACTGGCGAACCGT3'

Hind III

E2-down

5'GGATCCTTATACAAATGCAGACATATACCC3'

BamHI

E2N-down

5'GGATCCTTATGATTCTGCTGAGGC3'

BamHI

E2C-up

5'CTTAAGGTGGCTGGGACTGTTATTCAC3'

Hind III

118-up

5'CAGTACTAGTGAAATTTGATGGCAGC3'

\

118-down

5'GCTGCCATCAAATTTCACTAGTACTG3'

\

235-up

5'CAGAACCAACAGGAGCAGGAAG3'

\

235-down

5'GTGGAGGACACCCTGGCATAC3'

\

338-up

5'GTATGCCAGGGTGTCCTCCAC3'

\

338-down

5'GTGGAGGACACCCTGGCATAC3'

\

m338-up

5'GTATGTCAGGGTGTCCTCCAC3'

\

m338-down

5'GTGGAGGACACCCTGACATAC3'

\

E2-P-up

5'GGATCCATGGAAACACTGGCGAACCGT3'

BamHI

E2N-P-down

5'GAATTCTTATGATTCTGCTGAGGC3'

EcoRI

E2C-P-up

5'GGATCCGTGGCTGGGACTGTTATTCAC3'

BamHI

E2-P-down

5'GAATTCTTATACAAATGCAGACATATACCC3'

EcoRI

E2-P-117-up

5'GGATCCTACATCCGCATCTGTGTCTAG3'

BamHI