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Figure 5 | BMC Molecular Biology

Figure 5

From: A point mutation in the DNA-binding domain of HPV-2 E2 protein increases its DNA-binding capacity and reverses its transcriptional regulatory activity on the viral early promoter

Figure 5

Comparative analyses of DNA-binding activities between the HPV-2 prototype E2 (represented as E2) and mutated E2 at the position of residual 338 (represented as E2-A338V). A: oligo HPV-E2BS; B: oligo HPV-2 prototype; C: oligo HPV-16 prototype. Equally molar amounts (1.3 × 10-11 mol) of E2 and E2-A338V proteins in three different constructs were mixed the individual biotin-labeled probes and the protein-DNA complexes were separated in 6.5% PAGE gels. Lane 1 and 4: E2-FL protein; Lane 2 and 5: E2-HC; Lane 3 and 6: E2-C. The probe amounts used in EMSA are indicated at the bottom of the graphs and the protein amounts are shown above the graphs. The protein-DNA complexes of the preparations are indicated by arrows marked E2-C (lane 1 and 4), E2-HC (lane 2 and 5) and E2-FL (lane 3 and 6), respectively. F: free probe

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