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Figure 3 | BMC Molecular Biology

Figure 3

From: A point mutation in the DNA-binding domain of HPV-2 E2 protein increases its DNA-binding capacity and reverses its transcriptional regulatory activity on the viral early promoter

Figure 3

Expressions and purifications of various recombinant HPV-2 E2 proteins. A. Schematic structures of the full-length E2 and various mutated E2. The mutations are marked by the black crosses. B. 15% SDS-PAGE assays of various purified recombinant E2 proteins. Full length and various mutated E2 protein with GST tag were expressed in E. coli BL21 and purified with Glutathione sepharose 4B Agarose. 0.5 μg of each purified E2 protein was loaded on 15% SDS-PAGE and stained by coomassie brilliant blue. Each purified protein is indicated on the tops of the graphs. M: Protein molecular markers. The molecular sizes are shown on the left

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