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Figure 1 | BMC Molecular Biology

Figure 1

From: A point mutation in the DNA-binding domain of HPV-2 E2 protein increases its DNA-binding capacity and reverses its transcriptional regulatory activity on the viral early promoter

Figure 1

Expressions of various HPV-2 E2 proteins in the cultured cells. A. Schematic structures of the full-length E2 and various mutated E2. The black crosses indicate the amino acid exchanges of these mutants. B. HeLa (left panel) or C33A (right panel) cells were either mock transfected or transfected with 500 ng of pcDNA-E2-proto or pcDNA-E2-A338V as indicated. Cells were harvested 24, 48 or 72 h post-transfection. The prepared cells extracts were separated by 15% SDS-PAGE, transferred to nitrocellulose membrane and probed with E2 or β-actin antibody as indicated. Exposition time was 5 min for E2 and 2 min for β-actin. The blot shown is a representative experiment among three experiments. C. 500 ng of various E2 constructs were co-transfected with 2 μg CAT reporter plasmid pCAT-LCR into HeLa cells, respectively. Cells were harvested 48 h post-transfection. The blot shown is a representative experiment among three experiments. The blots of E2, E2-N and β-actin are indicated on the left and relative molecular weights are arrowed on right

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