Figure 4

P53 is required for NF-κB-mediated miR-34a induction. (a)KYSE450 cells were transfected either with empty vector (pcDNA3.1) or with p65 expression vector (p65), 48 h after transfection, levels of p65 and mature miR-34a were individually detected by western blot or qRT-PCR. (b)KYSE450 cells were cotransfected with wildtype p53 or control vectors and p65 expression vectors or pCDNA3.1, 48 h after transfection, levels of p53 and mature miR-34a were individually detected by western blot or qRT-PCR. (c) pCDNA3.1or p65 and siRNA control or sip53 were cotransfected into EC109 cells. P53 protein and mature miR-34a levels were individually detected by western blot or qRT-PCR 48 h after transfection.