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Figure 5 | BMC Molecular Biology

Figure 5

From: Alternative splicing of the neurofibromatosis type 1 pre-mRNA is regulated by the muscleblind-like proteins and the CUG-BP and ELAV-like factors

Figure 5

MBNL and CELF proteins bind strongly to wild-type NF1 pre-mRNA, but not to mutant pre-mRNA. (A) Sequences of RNA oligonucleotides. Potential MBNL sites are underlined. Mutations are presented in italics. The nucleotide positions relative to exon 23a are indicated. (B) End labeled RNA oligonucleotides were incubated with either GST as a control (750 ng) or GST-MBNL1 (750 ng), and binding was detected by UV cross-linking assays. (C) End labeled RNA oligonucleotides were incubated with either GST (100 ng) or GST-CELF2 (100 ng), and binding was detected by UV cross-linking assays.

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BMC Molecular Biology

ISSN: 1471-2199

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