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Figure 3 | BMC Molecular Biology

Figure 3

From: Directional telomeric silencing and lack of canonical B1 elements in two silencer Autonomously Replicating Sequences in S. cerevisiae

Figure 3

Proto-silencer and replicator activity of ARS317 and ARS319 . A. The ARSs and their mutant derivatives (shown on the left) were integrated in the VIIL telomere in URA3-ACS-B1-tel direction. The putative WTW motifs are underlined. The mutations are depicted by small letters. The silencer activity of the constructs was assessed as % FOAR cells and plotted. Data is from Table1. The p values for all constructs are above 0.05 and are not shown. B. The ARSs and their mutant derivatives were cloned between CEN4 and URA3 to produce mini-chromosomes. All mini-chromosomes were transformed in W303 cells and loss per generation was calculated and plotted. Data is from Table1.

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