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Figure 4 | BMC Molecular Biology

Figure 4

From: Cloning, ligand-binding, and temporal expression of ecdysteroid receptors in the diamondback moth, Plutella xylostella

Figure 4

In vitro transcription-translation of P. xylostella ecdysone receptors. Receptors were cloned into pF25 T7 Flexi Vector and expressed using TNT T7 Insect Cell Extract Protein Expression system. Proteins were synthesized in the presence of Transcend™ tRNA, separated on a 10% SDS–PAGE gel, and visualized by the colorimetric detection using streptavidin-alkaline phosphatase. EcRs, USP, Luciferase (positive control), and a negative control were visualized with/without gel band of the predicted size (PxEcRA: 57kDa, PxEcRB: 62kDa, PxUSP: 47kDa, Luciferase: 61kDa, respectively).

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