Analysis of gene transcripts in fat body of the fifth instar molting larvae after AK2 knockdown by feeding dsRNA to newly hitched larvae. A. Semi-quantitative RT-PCR, Lane 1, dsGFP- feeding larvae; lane 2, dsAK2-feeding larvae. B. Statistical analysis of results in A. The asterisk indicates significant difference from the control (p < 0.05, n = 3) by student t test.