Immunohistochemistry showing the co-localization of AK2 with mitochondria. a. Detection of AK2 in the cells with the rabbit polyclonal antiserum against Helicoverpa AK2 and the goat anti-rabbit-Alexa Fluor 488, the green fluorescence indicates AK2, the column in panel a is the Western blot showing the specificity of the antibody against Helicoverpa AK2 using fat body from the 5th instar feeding larvae as the protein sample. b. Detection of mitochondria with MitoTracker, red fluorescence indicates mitochondria. c. DAPI-stained nucleus, blue fluorescence indicates the nucleus. d. Merging of images in a, b, and c, the orange color is caused by the overlap of green-colored AK2 and red-colored mitochondria. e. Preserum as a negative control for the antiserum against AK2. f. The negative control without MitoTracker. Bar is 20 μm.