Skip to main content
Figure 6 | BMC Molecular Biology

Figure 6

From: The transcriptional activator ZNF143 is essential for normal development in zebrafish

Figure 6

SPH sites in the zebrafish pax2a promoter are functional for transcription in transfected ZF4 cells. Zebrafish ZF4 cells were transfected per well with 200 ng plasmid DNA containing wt or SPHMUT pax2a promoters driving firefly luciferase expression, plus 200 ng pRL-SV40 renilla luciferase plasmid as a normalization control, plus 25 ng of either empty vector pCI-basic plasmid (unfilled columns) or pCI/myczZNF143 to express myc-tagged full-length ZNF143 (filled columns). The ratio of luciferase/renilla light units was determined for each sample, and these ratios were compared relative to the wt promoter with no transfected ZNF143 plasmid included in every experiment. The height of each column represents the average value for the number of independently transfected samples (specified in parentheses), and error bars depict one standard deviation above and below the mean. A double asterisk signifies a p-value <0.01, and a single asterisk signifies a p-value <0.05 comparing like reporter samples with and without addition of ZNF143 expression plasmid.

Back to article page