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Figure 4 | BMC Molecular Biology

Figure 4

From: A novel 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) splice variant with an alternative exon 1 potentially encoding an extended N-terminus

Figure 4

Promoter activities of the canonical HMGCR-1a and the putative HMGCR-1b promoter in HepG2 cells. Solid bars illustrate different sizes of genomic sequences upstream of exon 1a and exon 1b that were cloned into pGL4.10. The HMGCR promoter SNP (C > A, rs3761740) is highlighted in red and represented by both alleles. Luciferase activity was measured in the lysates of transfected cells incubated for 24 h in fetal bovine serum (FBS), lipoprotein-deficient serum (LPDS) or LPDS with 10 μM atorvastatin (Atv). The promoter activity is shown as the mean fold-change in firefly luciferase activity relative to the mean firefly luciferase activity of the empty pGL4.10 vector, which was set to 1 after correction for transfection efficiency using Renilla luciferase activity. The results are the mean of three independent experiments performed in triplicate. Error bars indicate standard errors (± S.E).

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BMC Molecular Biology

ISSN: 1471-2199

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