Figure 4

(A) Diagram of internal-terminal deletions of OXR1 (8–13). (B) Papillation assays with plasmids expressing OXR1 internal deletions in MV4709 containing LacI-producing plasmid pMS421. Dilutions and cell platings were as in Figure 2B using plates containing 1.0 mM IPTG on minimal lactose plates containing 100 μg/ml carbenicillin, 40 μg/ml streptomycin and 20 μg/ml spectinomycin. (C) SDS-PAGE of extracts containing plasmids expressing OXR1 internal deletions.