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Table 1 Substrates

From: Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase – engineering a thermostable ATP independent enzyme

Acceptor substrates:

 

UCC UAU GAU GCA GGC CUU ACU AGG UGC AGU

(RNA30)

UCC UAU GAU GCA GGC CUU ACU AGG UGC AGU-OCH3

(RNA30-OMe)

TCC TAT GAT GCA GGC CTT ACT AGG TGC AGT

(DNA30)

CAT TGT AGC CGT CCA TCT TTT CCT C

(DNA25)

Donor substrates:

 

pCTG TAG GCA CCA TCA AT-NH2

(pDNA17-NH2)

AppCTG TAG GCA CCA TCA AT-NH2

(AppDNA17-NH2)

AppTCG TAT GCC GTC TTC TGC TTG-NH2

(AppDNA21-NH2)

AppUCG UAU GCC GUC UUC UGC UUG-NH2

(AppRNA21-NH2)

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BMC Molecular Biology

ISSN: 1471-2199

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