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Table 7 Comparison of the relative mRNA expression levels* of four target genes in fluconazole-treated C. glabrata when normalized to different reference genes

From: Evaluation of reference genes for real-time quantitative PCR studies in Candida glabrata following azole treatment

Gene CDR1 PDR1 ERG4 ERG10
ACT1 0.26 0.27 0.70 1.21
RDN5.8 4.07 4.27 11.08 19.07
PGK1 1.90 1.99 5.17 8.90
UBC13 2.23 2.33 6.06 10.43
RDN5.8+PGK1 2.79 2.92 7.59 13.06
RDN5.8+UBC13 3.01 3.15 8.19 14.09
RDN5.8+PGK1+UBC13 2.58 2.71 7.03 12.10
  1. *Values indicate the fold change in RNA transcription for each target gene in fluconazole-treated C. glabrata after normalization to one or more reference genes, as compared with untreated cells.
  2. ΔCT = CTtarget − CTreference.
  3. ΔΔCT(t) = ΔCT(t) − ΔCT(ut); ut, untreated; t, fluconazole-treated. Fold change = 2-ΔΔCT(t).
  4. Fold changes of the target genes were also computed with the hkgFinder tool by using one or more reference genes as the internal control for normalization
  5. P <; 0.05 vs. untreated cells, for all values shown in the table after normalizing to one or more reference genes as indicated.