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Table 6 Comparison of fluconazole-induced target gene expression among five different C. glabrata strains using RDN5.8 or ACT1 as the reference for normalization

From: Evaluation of reference genes for real-time quantitative PCR studies in Candida glabrata following azole treatment

  CDR1 PDR1 ERG4
Strain RDN5.8 ACT1 RDN5.8 ACT1 RDN5.8 ACT1
NCCLS84 2.12* 0.19* 2.22* 0.20* 2.85* 0.26*
Cg84u 3.90* 0.42* 3.26* 0.50* 10.04* 0.68*
CgB4 0.64# 0.06* 1.08# 0.10* 2.42* 0.22*
Cg3S 2.59* 0.23* 3.31* 0.30* 3.84* 0.35*
Cg4R 2.14* 0.19* 3.68* 0.33* 5.34* 0.48*
  1. Values indicate the fold change in RNA transcription for each target gene in fluconazole-treated C. glabrata, as compared with untreated cells.
  2. ΔCT = CTtarget − CTreference; ΔCT of each target gene was calculated by using RDN5.8 or ACT1 as the reference.
  3. ΔΔCT(t) = ΔCT(t) − ΔCT(ut); ut, untreated; t, fluconazole-treated. Fold change = 2-ΔΔCT(t).
  4. Fold changes of the target genes were also computed with the hkgFinder tool by using RDN5.8 or ACT1 as the reference.
  5. #P > 0.05 and *P <; 0.05 for the azole-treated group vs. the untreated group after normalization to RDN5.8 or ACT1.