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Table 6 Comparison of fluconazole-induced target gene expression ¶ among five different C. glabrata strains using RDN5.8 or ACT1 as the reference for normalization

From: Evaluation of reference genes for real-time quantitative PCR studies in Candida glabrata following azole treatment

 

CDR1

PDR1

ERG4

Strain

RDN5.8

ACT1

RDN5.8

ACT1

RDN5.8

ACT1

NCCLS84

2.12*

0.19*

2.22*

0.20*

2.85*

0.26*

Cg84u

3.90*

0.42*

3.26*

0.50*

10.04*

0.68*

CgB4

0.64#

0.06*

1.08#

0.10*

2.42*

0.22*

Cg3S

2.59*

0.23*

3.31*

0.30*

3.84*

0.35*

Cg4R

2.14*

0.19*

3.68*

0.33*

5.34*

0.48*

  1. ¶Values indicate the fold change in RNA transcription for each target gene in fluconazole-treated C. glabrata, as compared with untreated cells.
  2. ΔCT = CTtarget − CTreference; ΔCT of each target gene was calculated by using RDN5.8 or ACT1 as the reference.
  3. ΔΔCT(t) = ΔCT(t) − ΔCT(ut); ut, untreated; t, fluconazole-treated. Fold change = 2-ΔΔCT(t).
  4. Fold changes of the target genes were also computed with the hkgFinder tool by using RDN5.8 or ACT1 as the reference.
  5. #P > 0.05 and *P <; 0.05 for the azole-treated group vs. the untreated group after normalization to RDN5.8 or ACT1.
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BMC Molecular Biology

ISSN: 1471-2199

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