Evaluation of reference genes for real-time quantitative PCR studies in Candida glabrata following azole treatment

Table 4 **Comparison of reference gene expression stability* as determined by four different software packages**

geNorm		BestKeeper		NormFinder		hkgFinder
Gene	Mean M	Gene	r	Gene	Stability value	Gene	Fold change
RDN18	0.042	UBC7	0.983	UBC7	0.425	RDN18	1.2
RDN25	0.050	UBC13	0.978	PGK1	0.459	RDN25	1.3
RDN5.8	0.055	PGK1	0.974	UBC13	0.460	RDN5.8	1.4
UBC13	0.357	RDN5.8	0.915	PPIA	0.534	UBC13	2.5
PGK1	0.478	RDN25	0.908	RDN5.8	0.922	PGK1	2.9
UBC7	0.500	RDN18	0.901	RDN25	0.935	UBC7	3.0
PPIA	0.849	PPIA	0.864	RDN18	0.961	PPIA	8.7
RPL13A	1.103	RPL13A	0.857	RPL13A	0.993	RPL13A	18.0
ACT1	1.167	SDHA	0.856	ACT1	1.200	ACT1	21.8
SDHA	1.258	ACT1	0.836	SDHA	1.480	SDHA	32.7

* Genes are listed from most to least stable for all four methods.
M, geNorm stability parameter. Mean M , stability value.
r , all 10 candidate reference genes were correlated and were combined to calculate the BestKeeper index, which was then used to determine the correlation between each reference gene and the index.

ISSN: 1471-2199