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Table 4 Comparison of reference gene expression stability* as determined by four different software packages

From: Evaluation of reference genes for real-time quantitative PCR studies in Candida glabrata following azole treatment

geNorm BestKeeper NormFinder hkgFinder
Gene Mean M Gene r Gene Stability value Gene Fold change
RDN18 0.042 UBC7 0.983 UBC7 0.425 RDN18 1.2
RDN25 0.050 UBC13 0.978 PGK1 0.459 RDN25 1.3
RDN5.8 0.055 PGK1 0.974 UBC13 0.460 RDN5.8 1.4
UBC13 0.357 RDN5.8 0.915 PPIA 0.534 UBC13 2.5
PGK1 0.478 RDN25 0.908 RDN5.8 0.922 PGK1 2.9
UBC7 0.500 RDN18 0.901 RDN25 0.935 UBC7 3.0
PPIA 0.849 PPIA 0.864 RDN18 0.961 PPIA 8.7
RPL13A 1.103 RPL13A 0.857 RPL13A 0.993 RPL13A 18.0
ACT1 1.167 SDHA 0.856 ACT1 1.200 ACT1 21.8
SDHA 1.258 ACT1 0.836 SDHA 1.480 SDHA 32.7
  1. * Genes are listed from most to least stable for all four methods.
  2. M, geNorm stability parameter. Mean M , stability value.
  3. r , all 10 candidate reference genes were correlated and were combined to calculate the BestKeeper index, which was then used to determine the correlation between each reference gene and the index.