Skip to main content

Table 3 Assessment of reference gene expression stability in fluconazole-treated C. glabrata by using hkgFinder

From: Evaluation of reference genes for real-time quantitative PCR studies in Candida glabrata following azole treatment

  RDN18 RDN25 RDN5.8 UBC13 PGK1 UBC7 GAPDH UBC4 TUB1 EF1α PPIA RPL2A RPL10 RPL13A ACT1 SDHA
SD 0.19 0.22 0.26 0.73 0.87 0.88 1.19 1.33 1.42 1.58 1.71 2.01 2.26 2.28 2.44 2.76
Log fold change -0.35 -0.39 -0.46 -1.33 -1.56 -1.60 -2.18 -2.41 -2.60 -2.89 -3.12 -3.67 -4.12 -4.17 -4.45 -5.03
Fold change 1.2 1.3 1.4 2.5 2.9 3.0 4.5 5.3 6.0 7.4 8.7 12.7 17.4 18.0 21.8 32.7
Ranking 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
  1. The fold change shown in the table represents the difference in reference gene expression between azole-treated and untreated C. glabrata without normalization to an internal control gene.
  2. The stability ranking is based on the values of standard deviation (SD), log fold change, and fold change of reference genes in fluconazole-treated C. glabrata cells. The best reference genes will have the smallest SD and smallest fold-change values.