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Table 3 Assessment of reference gene expression stability in fluconazole-treated C. glabrata by using hkgFinder

From: Evaluation of reference genes for real-time quantitative PCR studies in Candida glabrata following azole treatment

 

RDN18

RDN25

RDN5.8

UBC13

PGK1

UBC7

GAPDH

UBC4

TUB1

EF1α

PPIA

RPL2A

RPL10

RPL13A

ACT1

SDHA

SD

0.19

0.22

0.26

0.73

0.87

0.88

1.19

1.33

1.42

1.58

1.71

2.01

2.26

2.28

2.44

2.76

Log fold change

-0.35

-0.39

-0.46

-1.33

-1.56

-1.60

-2.18

-2.41

-2.60

-2.89

-3.12

-3.67

-4.12

-4.17

-4.45

-5.03

Fold change

1.2

1.3

1.4

2.5

2.9

3.0

4.5

5.3

6.0

7.4

8.7

12.7

17.4

18.0

21.8

32.7

Ranking

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

  1. The fold change shown in the table represents the difference in reference gene expression between azole-treated and untreated C. glabrata without normalization to an internal control gene.
  2. The stability ranking is based on the values of standard deviation (SD), log fold change, and fold change of reference genes in fluconazole-treated C. glabrata cells. The best reference genes will have the smallest SD and smallest fold-change values.
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BMC Molecular Biology

ISSN: 1471-2199

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